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As the 16S profiles within the gut show up really various for intracellular and extracellular DNA [33], qPCR is expected to reflect the NGS-specific Neighborhood structure the two quantitatively and qualitatively additional closely than circulation cytometry.

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In conclusion, we warning from the Examination of microbiome NGS information exclusively relying on relative abundance, considering the fact that compositionality could skew biological inferences from microbiome experiments per

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The 331F/797R primers were being chosen for your quantification of complete germs, given that the primers goal the V3-V4 hypervariable areas as in Illumina MiSeq. A list of primers and references used in the current analyze is summarized in S1 Desk.

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